Biotechnology A Laboratory Course

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Détails bibliographiques
Auteur principal: Becker, Jeffrey M. (19..-....). (Auteur)
Autres auteurs: Caldwell, Guy A.. (Auteur)
Support: E-Book
Langue: Anglais
Publié: San Diego, CA : Elsevier Science, 1996.
Sujets:
Autres localisations: Voir dans le Sudoc
Résumé: The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences. Key Features of this new edition: * All exercises and appendixes have been updated * Includes new exercises on * Polymerase chain reaction * Beta-Galactosidase detection in yeast colonies * Western blotting * New procedures introduced for * Large-scale plasmid isolation * Yeast transformation * DNA quantitation * New appendixes added, one of which provides details on accessing biological information sites on the Internet (World Wide Web) * Use of non-radioactive materials and easy access to microbial cultures * Laboratory exercises student tested for seven years
Accès en ligne: Accès à l'E-book
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Résumé:The objectives of this Second Edition of Biotechnology: A Laboratory Course remain unchanged: to create a text that consists of a series of laboratory exercises that integrate molecular biology with protein biochemistry techniques while providing a continuum of experiments. The course begins with basic techniques and culminates in the utilization of previously acquired technical experience and experimental material. Two organisms, Sacchaomyces cerevisiae and Escherichia coli, a single plasmid, and a single enzyme are the experimental material, yet the procedures and principles demonstrated are widely applicable to other systems. This text will serve as an excellent aid in the establishment or instruction of introductory courses in the biological sciences. Key Features of this new edition: * All exercises and appendixes have been updated * Includes new exercises on * Polymerase chain reaction * Beta-Galactosidase detection in yeast colonies * Western blotting * New procedures introduced for * Large-scale plasmid isolation * Yeast transformation * DNA quantitation * New appendixes added, one of which provides details on accessing biological information sites on the Internet (World Wide Web) * Use of non-radioactive materials and easy access to microbial cultures * Laboratory exercises student tested for seven years
Description:Titre provenant de la page de titre du document numérique.
Numérisation de l'édition de San Diego, CA, USA : Elsevier Science & Technology Books, 1996.
La pagination de l'édition imprimée correspondante est de 282 p.
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ISBN:9780080528199
0080528198 (electronic bk)
Accès:L'accès complet à la ressource est réservé aux usagers des établissements qui en ont fait l'acquisition